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anti lepr rabbit polyclonal antibody  (Proteintech)


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    Structured Review

    Proteintech anti lepr rabbit polyclonal antibody
    Anti Lepr Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+lepr+rabbit+polyclonal+antibody/LEPR+Antibody/pm41066485-150-31-36
    Average 94 stars, based on 36 article reviews
    anti lepr rabbit polyclonal antibody - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Staining:

    Article Title: In Vivo Evaluation of Bone Regenerative Capacity of the Novel Nanobiomaterial: β-Tricalcium Phosphate Polylactic Acid-co-Glycolide (β-TCP/PLLA/PGA) for Use in Maxillofacial Bone Defects.
    Article Snippet: .. Primary antibody staining was performed with anti-Runx2 rabbit polyclonal antibody (abcam: ab23981; 1:300 conc), anti-LEPR rabbit polyclonal antibody (Proteintech, 20966-1-AP; 1:150 conc), anti-human osteocalcin monoclonal antibody clone (BIO-RAD, 0400-0041; 1:3000 conc), and anti-Periostin rabbit polyclonal antibody (abcam: ab14041; 1:800 conc) at room temperature for 50 min. After another PBS wash, secondary antibody staining was performed with Histofine Simple Stain Rat MAX-PO (MULTI) (Nichirei Bioscience Inc., Tokyo, Japan 414191) at room temperature for 30 min. After another PBS Nanomaterials 2024, 14, 91 8 of 27 wash, the slides were DAB-colored for 10 min and finally stained with Meyer’s hematoxylin for 30 s. The coloration was removed, a water wash was performed, and the specimencontaining slides underwent dehydration, permeabilization, and sealing. ..

    Article Title: Feasibility of Application of the Newly Developed Nano-Biomaterial, β-TCP/PDLLA, in Maxillofacial Reconstructive Surgery: A Pilot Rat Study
    Article Snippet: .. Nonspecific staining was blocked by incubating the sections in 5% bovine serum albumin (BSA) in PBS for 1 h. Endogenous peroxidase activity was quenched by incubating the sections in a solution of 3% H 2 O 2 in 10% methanol and PBS for 5 min. After washing with PBS, the sections were incubated with the following primary antibodies at room temperature for 50 min: anti-Runx2 rabbit polyclonal antibody (ab23981, 1:1000 dilution; Abcam, Tokyo, Japan); anti-OCN mouse monoclonal antibody (ab13420, 1:750 dilution; OCG3, Abcam); anti-LepR rabbit polyclonal antibody (20966-1-AP, 1:200 dilution; Proteintech, Rosemont, IL, USA). ..

    Article Title: In Vivo Evaluation of Bone Regenerative Capacity of the Novel Nanobiomaterial: β-Tricalcium Phosphate Polylactic Acid-co-Glycolide (β-TCP/PLLA/PGA) for Use in Maxillofacial Bone Defects
    Article Snippet: .. Primary antibody staining was performed with anti-Runx2 rabbit polyclonal antibody (abcam: ab23981; 1:300 conc), anti-LEPR rabbit polyclonal antibody (Proteintech, 20966-1-AP; 1:150 conc), anti-human osteocalcin monoclonal antibody clone (BIO-RAD, 0400-0041; 1:3000 conc), and anti-Periostin rabbit polyclonal antibody (abcam: ab14041; 1:800 conc) at room temperature for 50 min. After another PBS wash, secondary antibody staining was performed with Histofine Simple Stain Rat MAX-PO (MULTI) (Nichirei Bioscience Inc., Tokyo, Japan 414191) at room temperature for 30 min. After another PBS wash, the slides were DAB-colored for 10 min and finally stained with Meyer’s hematoxylin for 30 s. The coloration was removed, a water wash was performed, and the specimen-containing slides underwent dehydration, permeabilization, and sealing. ..

    Incubation:

    Article Title: Evaluation of bone regenerative and bioactive potential of β-TCP/PLLA/PGA nanobiomaterial in conjunction with concentrated conditioned media from rapidly expanding clone (REC) of mesenchymal stem cells: A pilot animal study.
    Article Snippet: .. Primary antibody incubation was performed at room temperature for 50 min using the following specific antibodies: anti-Runx2 rabbit polyclonal antibody (1:300; Abcam: ab23981), anti-human osteocalcin monoclonal antibody clone (1:3000; BIO-RAD 0400−0041), anti-LepR rabbit polyclonal antibody (1:150; Proteintech, 20966–1-AP), and anti-periostin rabbit polyclonal antibody (1:800; Abcam: ab14041). .. Sections were washed with PBS and incubated with Histofine Simple Stain Rat MAX-PO (MULTI) (Nichirei Biosciences Inc., Tokyo, Japan; 414191) secondary antibodies for 30 min. A diaminobenzidine (DAB) substrate was used to visualize antigen-antibody binding, resulting in a brown precipitate, which was then counterstained with Meyer’s hematoxylin, dehydrated, and mounted for analysis.

    Article Title: Feasibility of Application of the Newly Developed Nano-Biomaterial, β-TCP/PDLLA, in Maxillofacial Reconstructive Surgery: A Pilot Rat Study
    Article Snippet: .. Nonspecific staining was blocked by incubating the sections in 5% bovine serum albumin (BSA) in PBS for 1 h. Endogenous peroxidase activity was quenched by incubating the sections in a solution of 3% H 2 O 2 in 10% methanol and PBS for 5 min. After washing with PBS, the sections were incubated with the following primary antibodies at room temperature for 50 min: anti-Runx2 rabbit polyclonal antibody (ab23981, 1:1000 dilution; Abcam, Tokyo, Japan); anti-OCN mouse monoclonal antibody (ab13420, 1:750 dilution; OCG3, Abcam); anti-LepR rabbit polyclonal antibody (20966-1-AP, 1:200 dilution; Proteintech, Rosemont, IL, USA). ..

    Article Title: Evaluation of bone regenerative and bioactive potential of β-TCP/PLLA/PGA nanobiomaterial in conjunction with concentrated conditioned media from rapidly expanding clone (REC) of mesenchymal stem cells: A pilot animal study
    Article Snippet: .. Primary antibody incubation was performed at room temperature for 50 min using the following specific antibodies: anti-Runx2 rabbit polyclonal antibody (1:300; Abcam: ab23981), anti-human osteocalcin monoclonal antibody clone (1:3000; BIO-RAD 0400−0041), anti-LepR rabbit polyclonal antibody (1:150; Proteintech, 20966–1-AP), and anti-periostin rabbit polyclonal antibody (1:800; Abcam: ab14041). .. Sections were washed with PBS and incubated with Histofine Simple Stain Rat MAX-PO (MULTI) (Nichirei Biosciences Inc., Tokyo, Japan; 414191) secondary antibodies for 30 min. A diaminobenzidine (DAB) substrate was used to visualize antigen-antibody binding, resulting in a brown precipitate, which was then counterstained with Meyer’s hematoxylin, dehydrated, and mounted for analysis.

    Activity Assay:

    Article Title: Feasibility of Application of the Newly Developed Nano-Biomaterial, β-TCP/PDLLA, in Maxillofacial Reconstructive Surgery: A Pilot Rat Study
    Article Snippet: .. Nonspecific staining was blocked by incubating the sections in 5% bovine serum albumin (BSA) in PBS for 1 h. Endogenous peroxidase activity was quenched by incubating the sections in a solution of 3% H 2 O 2 in 10% methanol and PBS for 5 min. After washing with PBS, the sections were incubated with the following primary antibodies at room temperature for 50 min: anti-Runx2 rabbit polyclonal antibody (ab23981, 1:1000 dilution; Abcam, Tokyo, Japan); anti-OCN mouse monoclonal antibody (ab13420, 1:750 dilution; OCG3, Abcam); anti-LepR rabbit polyclonal antibody (20966-1-AP, 1:200 dilution; Proteintech, Rosemont, IL, USA). ..



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